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Image Search Results
Journal: Plastic and Reconstructive Surgery
Article Title: Extracellular Vesicles from Adipose-Derived Stem Cells Relieve Extremity Lymphedema in Mouse Models
doi: 10.1097/PRS.0000000000010388
Figure Lengend Snippet: ADSC-EVs increase lymphangiogenesis and angiogenesis and decrease fibrosis in the lymphedema limb. ( Above , left ) Representative 40× images of sections from control and ADSC-EV–treated limbs 6 weeks after surgery with the immunofluorescence localization of LYVE-1 + and CD31 + cells. ( Above , right ) Quantification of the LYVE-1 + CD31 + overlap areas per 0.25 mm 2 (four random areas per limb) after treatment with PBS, HEK293-EVs, or ADSC-EVs. All the data are presented as the mean ± SEM, with P < 0.05 considered as significant (compared with the PBS group). Scale bars = 100 μm. ( Below , left ) Representative 20× images of limb sections from control and ADSC-EV–treated limbs 6 weeks after surgery with immunofluorescence localization of collagen 1, Hoechst, and CD31. ( Below , right ) Quantification of collagen 1–positive cells per 0.25 mm 2 (four random areas per limb) after treatment with PBS, HEK293-EVs, or ADSC-EVs. All the data are presented as the mean ± SEM, with P < 0.05 considered as significant (compared with the PBS group). Scale bars = 100 μm.
Article Snippet: The tissues were stained with Hoechst 33342 (Dojindo, Tokyo, Japan), lymphatic vessel endothelial receptor-1 (LYVE-1) polyclonal antibodies (bs-1311R-A555; Bioss Antibodies),
Techniques: Control, Immunofluorescence
Journal: Biomedicines
Article Title: Sodium Accumulation and Blood Capillary Rarefaction in the Skin Predispose Spontaneously Hypertensive Rats to Salt Sensitive Hypertension
doi: 10.3390/biomedicines10020376
Figure Lengend Snippet: Representative images of skin blood and lymphatic capillaries. ( A ). Anti-CD31/PECAM-1 immunohistochemistry. The antibody stains endothelial lining of dermal blood vessels (arrows). Original magnification ×40. ( B ). Anti-podoplanin immunohistochemistry. Primary antibody highlights dermal lymphatic vessels (arrows). Original magnification ×40.
Article Snippet: For immunohistochemical analysis, tissue sections (4 μm-thick) were pre-treated by heat mediated antigen retrieval and incubated with primary
Techniques: Immunohistochemistry